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D5546

Sigma-Aldrich

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

Numero MDL:
MFCD00217342
Codice UNSPSC:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Livello qualitativo

400

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Applicazioni

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

  • human osteosarcoma cells and HeLa cells
  • primary human mesenchymal stromal cells (hBMSCs)
  • human embryonic kidney cell lines

Ricostituzione

Supplement with 0.584 g/L glutamine.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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