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Sigma-Aldrich

Extract-N-Amp植物PCR试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

别名:

Plant direct PCR kit

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

品質等級

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

colorless

應用

agriculture

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

Extract-N-Amp 植物PCR试剂盒含有从植物叶片中快速提取基因组DNA并通过PCR反应扩增感兴趣的目标基因必需的所有试剂。使用新型Extraction Solution,无需通过液氮对植物组织进行传统的冰冻,机械式破碎、有机萃取或沉淀DNA操作。该试剂盒同时包含一种专为从提取物直接扩增而配制的PCR ReadyMix

基因组DNA提取自0.5至0.7 cm的植物叶盘,该叶盘通过标准打孔器获得并在95 oC下Extraction Solution中孵育10分钟。向提取物中加入等体积的Dilution Solution,以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix(包含在试剂盒内)的PCR反应物中。
Extract-N-Amp植物PCR试剂盒用于直接PCR(聚合酶链反应)。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案,无需常规的植物组织冷冻处理。

應用

Extract-N-Amp植物PCR试剂盒用于从植物和真菌菌丝体中分离基因组DNA,还用于聚合酶链式反应(PCR)。
Extract-N-Amp植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。

特點和優勢

  • 可在15分钟内一步提取出用于PCR反应的植物基因组DNA
  • 专为直接从提取物进行扩增而配置的PCR ReadyMix
  • 适用于基因组DNA的高度特异性PCR扩增的热启动抗体
  • 提取物可在4 °C 下至少稳定保存6个月
  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化,节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

成分

Extract-N-Amp植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物(2X)(缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体)。

其他說明

Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。
更多信息,请见www.sigma-aldrich.com/extract-n-amp

法律資訊

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
Ante Karoglan et al.
Acta dermato-venereologica, 99(13), 1253-1257 (2019-10-02)
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas
Obase K, et al.
Mycoscience (2021)
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and
José Dijair Antonino de Souza Júnior et al.
PloS one, 8(12), e85364-e85364 (2014-01-07)
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita

商品

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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