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Sigma-Aldrich

REDExtract-N-Amp™植物PCR试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

别名:

Plant direct PCR kit, Plant direct PCR kit with loading dye

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

品質等級

200

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

red

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

The REDExtract-N-Amp™植物PCR试剂盒能够通过直接PCR（聚合酶链反应）快速检测植物病原体。这些试剂盒提供了从植物叶片快速提取基因组DNA以及通过直接PCR扩增靶标所需的所有试剂。REDExtract-N-Amp PCR ReadyMix™含有染料，可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。

應用

REDExtract-N-Amp™ Plant PCR试剂盒已用于：
  • 从菌丝草坪和树叶中提取基因组DNA
  • 筛选推定转基因马铃薯植株的叶片组织
  • 进行聚合酶链式反应（PCR）
  • 从大麻种子和干果体鳃中分离DNA

特點和優勢

  • 一步提取PCR所需的植物基因组DNA，只需不到15分钟
  • 无需冷冻、机械破碎、有机萃取、柱纯化或液氮冷冻植物组织、机械破碎、有机提取、柱纯化或沉淀
  • 专门配制的PCR ReadyMix，与提取液配套使用
  • 热启动抗体支持高特异性PCR扩增基因组DNA
  • REDExtract-N-Amp™不需要凝胶分析所需的装载缓冲液或跟踪染料
  • 符合高通量植物基因分析要求
  • 提取物稳定：在4 °C环境下稳定至少6个月

成分

试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR Reaction Mix。

其他說明

有关其他信息，请参阅www.sigma-aldrich.com/extract-n-amp。

法律資訊

购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼，仅可将所购买的量用于购买者自己的内部研究。′其他专利权（例如5′核酸酶工艺的专利权）不得明示、暗示或禁止反言。 有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
根据美国专利号 5,338,671和5,587,287以及其他国家的相应专利获得抗体体外研究许可。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155和美国境外的声明，对应于过期的美国专利号5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Samuel J Harrison et al.
Plant methods, 2, 19-19 (2006-11-08)
The floral dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium is the method of choice for Arabidopsis transformation. The presence of a marker, usually antibiotic- or herbicide-resistance, allows identification of transformed seedlings from untransformed seedlings.
Siobhan M Brady et al.
Molecular systems biology, 7, 459-459 (2011-01-20)
Tightly controlled gene expression is a hallmark of multicellular development and is accomplished by transcription factors (TFs) and microRNAs (miRNAs). Although many studies have focused on identifying downstream targets of these molecules, less is known about the factors that regulate
Giuseppe Forlani et al.
Forensic science international. Genetics, 52, 102464-102464 (2021-01-19)
The availability of molecular markers able to distinguish drug-type from fiber-type Cannabis sativa cultivars would allow fast and cheap analysis of any plant specimen, including seeds and leaves. Several approaches to this issue have been described, mainly using polymorphisms in
Rebeca Cobos et al.
Applied and environmental microbiology, 81(18), 6474-6483 (2015-07-15)
Grapevine trunk fungal pathogens, such as Diplodia seriata and Phaeomoniella chlamydospora, can infect plants through pruning wounds. They cause grapevine trunk diseases and are involved in grapevine decline. Accordingly, the protection of pruning wounds is crucial for the management of
Martha E Trujillo et al.
The ISME journal, 4(10), 1265-1281 (2010-05-07)
Our current knowledge of plant-microbe interactions indicate that populations inhabiting a host plant are not restricted to a single microbial species but comprise several genera and species. No one knows if communities inside plants interact, and it has been speculated
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商品

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

REDExtract-N-Amp Plant PCR Kits Protocol

The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves.

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