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Sigma-Aldrich

Extract-N-Amp™植物PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

别名:

Plant direct PCR kit

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 1000 amplifications
 sufficient for 1000 extractions

特點

hotstart

技術

PCR: suitable

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

Extract-N-Amp™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。

應用

Extract-N-Amp™植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。
Extract-N-Amp™组织PCR试剂盒用于从新鲜蘑菇中提取DNA。

特點和優勢

  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化，节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

成分

Extract-N-Amp™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。

其他說明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。
Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。

法律資訊

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Antoine Lf Gady et al.
Plant methods, 5, 13-13 (2009-10-09)
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as
Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and
José Dijair Antonino de Souza Júnior et al.
PloS one, 8(12), e85364-e85364 (2014-01-07)
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita
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商品

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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