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Sigma-Aldrich

REDExtract-N-Amp™ 组织PCR试剂盒

sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

别名:

Tissue direct PCR kit, Tissue direct PCR kit with loading dye

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About This Item

EC號碼:
254-457-8
分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 10 reactions
10 reactions sufficient for 10 amplifications
10 reactions sufficient for 10 extractions
 sufficient for 100 reactions
100 reactions sufficient for 100 amplifications
100 reactions sufficient for 100 extractions
 sufficient for 1000 reactions
1000 reactions sufficient for 1000 amplifications
1000 reactions sufficient for 1000 extractions

品質等級

200

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

red

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

REDExtract-N-Amp™植物PCR试剂盒包含从鼠尾和其它动物组织、口腔拭子、毛干和唾液中快速提取和扩增基因组DNA所需的所有试剂。无需机械破碎、有机提取、柱纯化或DNA沉淀。该试剂盒还包括专门配制的热启动PCR ReadyMix™，可直接从提取物中扩增。该配方使用基于抗体的热启动，用于特异性扩增。REDExtract-N-Amp 组织 PCR 试剂盒中含有一种示踪染料，可方便地将PCR反应产物直接上样到琼脂糖凝胶上进行分析。

應用

REDExtract-N-Amp™ 组织PCR试剂盒已被用于DNA提取和聚合酶链反应（PCR）。

特點和優勢

  • 用于基因分型的快速基因组 DNA 分离的理想选择
  • 在 15 分钟内一步提取用于 PCR 的基因组 DNA
  • PCR ReadyMix，为提取物直接扩增而专门配制
  • 用于基因组 DNA 高特异性 PCR 扩增的热启动抗体
  • 提取物在4oC下可稳定保存至少 6 个月。
  • REDExtract-N-Amp™含有一种示踪染料，可将PCR反应产物方便地直接上样到琼脂糖凝胶上进行分析。

成分

试剂盒含有Tissue Preparation Solution、Extraction Solution和REDExtract-N-Amp PCR Reaction Mix。

其他說明

REDExtract-N-Amp组织PCR试剂盒为从各种细胞和组织中快速提取DNA以及通过PCR扩增靶标提供了所有必需试剂。 这种新型提取方法无需长时间的酶消化或均质化处理。 该试剂盒还包括特殊配方的热启动PCR反应混合物，可直接从提取物中扩增。 REDExtract-N-Amp PCR ReadyMix含有惰性染料，可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。
更多信息，请见www.sigma-aldrich.com/extract-n-amp。

原則

该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组DNA提取自在室温下的组织制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和REDExtract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。

法律資訊

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

應用

产品编号
说明
价格

N3910

中和液 B

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訊號詞

Danger

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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商品

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

Extract-N-Amp™ FFPE Tissue PCR Protocol

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

he REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

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