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Merck
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Key Documents

T3073

Sigma-Aldrich

组织制备溶液

30 mL sufficient for 1,000 extractions

别名:

Extract-N-Amp 组织制备溶液, REDExtract-N-Amp 组织制备溶液, SYBR® Green Extract-N-Amp 组织制备溶液, 直接PCR组织制备溶液

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About This Item

EC號碼:
分類程式碼代碼:
12352200
NACRES:
NA.55

品質等級

用途

30 mL sufficient for 1,000 extractions
sufficient for 1000 reactions

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

组织制备溶液适用于Extract-N-Amp 组织PCR试剂盒、REDExtract-N-Amp 组织PCR试剂盒、以及SYBR® Green Extract-N-Amp组织PCR试剂盒。本产品旨在对试剂盒中的试剂进行补充,这些试剂包含许多萃取剂。

應用

组织制备溶液已被用于:
  • 结合尾巴片段进行基因分型
  • 从斑马鱼胚胎/鳍组织中提取基因组 DNA
  • 从小鼠胚胎的耳标、尾尖和胚胎卵黄囊中提取DNA。
  • 从真菌孢子和菌丝体中提取 DNA

法律資訊

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
Titilayo D O Falade et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)
In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jessica Wallingford et al.
Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)
Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition

实验方案

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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