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Sigma-Aldrich

REDExtract-N-Amp™植物PCR试剂盒

sufficient for 10 extractions, sufficient for 10 amplifications

别名:

Plant direct PCR kit, Plant direct PCR kit with loading dye

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 10 amplifications
 sufficient for 10 extractions
 sufficient for 10 reactions

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

red

儲存溫度

−20°C

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相关类别

一般說明

The REDExtract-N-Amp™植物PCR试剂盒能够通过直接PCR（聚合酶链反应）快速检测植物病原体。这些试剂盒提供了从植物叶片快速提取基因组DNA以及通过直接PCR扩增靶标所需的所有试剂。REDExtract-N-Amp PCR ReadyMix™含有染料，可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。

應用

REDExtract-N-Amp™ Plant PCR Kit has been used to:
  • extract genomic DNA from mycelial lawns and leaves
  • screen leaf tissues from putative transgenic potato plants
  • perform polymerase chain reaction (PCR)

特點和優勢

  • 一步提取PCR所需的植物基因组DNA，只需不到15分钟
  • 无需冷冻、机械破碎、有机萃取、柱纯化或液氮冷冻植物组织、机械破碎、有机提取、柱纯化或沉淀
  • 专门配制的PCR ReadyMix，与提取液配套使用
  • 热启动抗体支持高特异性PCR扩增基因组DNA
  • REDExtract-N-Amp™不需要凝胶分析所需的装载缓冲液或跟踪染料
  • 符合高通量植物基因分析要求
  • 提取物稳定：在4 °C环境下稳定至少6个月

成分

试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR Reaction Mix。

其他說明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律資訊

购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼，仅可将所购买的量用于购买者自己的内部研究。′其他专利权（例如5′核酸酶工艺的专利权）不得明示、暗示或禁止反言。 有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
根据美国专利号 5,338,671和5,587,287以及其他国家的相应专利获得抗体体外研究许可。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155和美国境外的声明，对应于过期的美国专利号5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

分析证书(COA)

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Martha E Trujillo et al.
The ISME journal, 4(10), 1265-1281 (2010-05-07)
Our current knowledge of plant-microbe interactions indicate that populations inhabiting a host plant are not restricted to a single microbial species but comprise several genera and species. No one knows if communities inside plants interact, and it has been speculated
Sandra J Hey et al.
Plant biotechnology journal, 4(2), 219-229 (2006-12-21)
The regulation of phytosterol biosynthesis in seeds is of interest to biotechnologists because of the efficacy of dietary phytosterols in reducing blood cholesterol in humans. Mevalonate synthesis via 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) is a key step in phytosterol biosynthesis.
José F Cobo-Díaz et al.
Systematic and applied microbiology, 37(3), 177-185 (2013-11-26)
Genista versicolor is an endemic legume from Sierra Nevada National Park which constitutes one of the UNESCO-recognized Biosphere Reserves. In the present study, a collection of strains nodulating this legume was analysed in characteristic soils of this ecosystem. Most strains
Cynthia E Gallagher et al.
Plant physiology, 135(3), 1776-1783 (2004-07-13)
Despite ongoing research on carotenoid biosynthesis in model organisms, there is a paucity of information on pathway regulation operating in the grasses (Poaceae), which include plants of world-wide agronomic importance. As a result, efforts to either breed for or metabolically
Lorena C Garcia et al.
International journal of systematic and evolutionary microbiology, 60(Pt 2), 331-337 (2009-08-05)
A novel actinomycete, designated strain GUI 15(T), isolated from the root nodules of a Pisum sativum plant was characterized taxonomically by using a polyphasic approach. The 16S rRNA gene sequence of strain GUI 15(T) showed highest similarity to Micromonospora pattaloongensis
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商品

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

REDExtract-N-Amp Plant PCR Kits Protocol

The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves.

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