推荐产品
用途
sufficient for 350 purifications
品質等級
環保替代產品特色
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技術
DNA purification: suitable
環保替代類別
, Aligned
儲存溫度
15-25°C
正在寻找类似产品? 访问 产品对比指南
一般說明
GenElute植物基因组DNA小量制备试剂盒可以简单、方便的从各种植物中分离高纯度的DNA。GenElute试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
應用
纯化所得的高纯度DNA可直接供以下敏感的下游应用使用：
- PCR
- 限制性内切酶酶切
- 克隆
- Southern印迹
特點和優勢
- 起始材料：不超过100 mg的植物组织
- 预期得率：至多20 μg
- 洗脱体积：100 - 200 μl
- 所需时间：<40分钟
- 是否需要RNase处理：否
原則
在1小时内，可从至多100 mg新鲜组织或10 mg冻干材料中提取出微克级的DNA。首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。纯化所得DNA长度超过20 kb。
其他說明
更多信息，请见www.sigma-aldrich.com/genomicdna。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
推薦
訊號詞
Danger
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C
安全危害
儲存類別代碼
8A - Combustible corrosive hazardous materials
其他客户在看
Minal Mhatre
Methods in molecular biology (Clifton, N.J.), 11013, 435-453 (2012-11-28)
Pineapple (Ananas comosus L., Merr.) is a commercially important crop, grown in the tropical and subtropical regions. However, the crop is faced with postharvest damage and poor varietal and nutritional improvement. Being a vegetatively propagated crop, conventional breeding programs take
Catherine Ravel et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 112(4), 738-743 (2005-12-20)
A previous study in wheat (Triticum aestivum L.) identified two candidate genes controlling a quantitative trait locus (QTL) for high-molecular-weight glutenin subunit (HMW-GS) GluBx. These candidates were Glu-B1-1, the structural gene coding for Glu1Bx, and the B homoeologous gene coding
V Roussel et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 108(5), 920-930 (2003-11-14)
A set of 41 wheat microsatellite markers (WMS), giving 42 polymorphic loci (two loci on each chromosome), was used to describe genetic diversity in a sample of 559 French bread wheat accessions (landraces and registered varieties) cultivated between 1800 and
Jochen C Reif et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 111(5), 838-845 (2005-07-22)
It has been claimed that the system that delivers the products of plant breeding reduces the diversity of cultivated varieties leading to an increased genetic vulnerability. The main goal of our study was to monitor the temporal trends in genetic
Catherine Ravel et al.
Functional & integrative genomics, 6(4), 310-321 (2006-03-29)
Wheat prolamin-box binding factor (WPBF) was shown to be an activator of Triticum aestivum L. storage protein genes. Three homoeologous genes encoding this transcription factor were isolated from a bacterial artificial chromosome genomic library and sequenced. The genes all have
实验方案
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门