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應用
SIGMAFAST™ OPD (o-苯二胺二盐酸盐)片剂用作酶免疫测定(EIA)检测氧化物酶活性的可溶底物。OPD作为EIA底物是因为它有高灵敏度。 使用OPD的EIA可以在计时分析中读数或用稀释的酸性溶液终止反应,过后在读数。
SIGMAFAST OPD已用于唾液病毒样颗粒(VLP)结合测定。它还已用于开发检测大鼠血清样品中的利妥昔单抗的酶联免疫吸附测定(ELISA)板。
SIGMAFAST OPD已用于唾液病毒样颗粒(VLP)结合测定。它还已用于开发检测大鼠血清样品中的利妥昔单抗的酶联免疫吸附测定(ELISA)板。
特點和優勢
SIGMAFAST™ OPD片剂不需要另外的缓冲液或步骤来制备活性底物溶液。
注意
该底物吸湿且光敏感
重構
一片OPD片剂和一片尿素过氧化氢/缓冲液片剂,溶解在20ml水中,提供20ml即用底物。 如果按照该方法溶解片剂,得到的终浓度是0.4 mg/ml OPD, 0.4 mg/ml尿素过氧化氢和0.05 M磷酸柠檬酸盐,pH5.0。片剂是独立铝膜包装。
将每组片剂溶解在20mL去离子水中,就能生成即用型含有OPD和尿素过氧化氢缓冲溶液。
法律資訊
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
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