跳转至内容
Merck
所有图片(1)

Key Documents

D9905

Sigma-Aldrich

Denhardt′s溶液

for Northern and Southern blotting, lyophilized powder

别名:

用于印迹技术的Denhardt′s 溶液

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

品質等級

100

形狀

lyophilized powder

技術

Southern blotting: suitable

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

2-8°C

相关类别

一般說明

Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。

應用

Denhardt溶液已被用于：
  • 作为杂交缓冲液的组分，用于脑切片的杂交
  • 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
  • 用于杂交混合物的制备

适合用于Southern和Northern印迹膜的封闭缓冲液。

生化／生理作用

Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。

成分

每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。

準備報告

制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Alterations in m-RNA expression for Cu, Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson?s disease
Kunikowska G and Jenner P
Brain Research, 968(2), 206-218 (2003)
Nancy Stralis-Pavese et al.
Nature protocols, 6(5), 609-624 (2011-04-30)
Microbial diagnostic microarrays (MDMs) are highly parallel hybridization platforms containing multiple sets of immobilized oligonucleotide probes used for parallel detection and identification of many different microorganisms in environmental and clinical samples. Each probe is approximately specific to a given group
R E Kaye et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(18), 8542-8546 (1992-09-15)
The inhibitory effects of cyclosporin A (CsA) and FK506 on Fc epsilon receptor type I-initiated increases in cytokine mRNA and the expression of their intracellular binding proteins were studied in interleukin 3 (IL-3)-dependent, mouse bone marrow-derived mast cells (BMMCs). In
M Simpson-Holley et al.
Journal of virology, 85(4), 1581-1593 (2010-12-15)
Vaccinia virus (VV) mutants lacking the double-stranded RNA (dsRNA)-binding E3L protein (ΔE3L mutant VV) show restricted replication in most cell types, as dsRNA produced by VV activates protein kinase R (PKR), leading to eIF2α phosphorylation and impaired translation initiation. Here
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门