推荐产品
生物源
sheep
品質等級
共軛
peroxidase conjugate
抗體表格
F(ab′)2 fragment of affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
lyophilized (clear, brown solution after reconstitution)
包裝
pkg of 150 U
製造商／商標名
Roche
儲存溫度
2-8°C
一般說明
地高辛是一种半抗原，可用于核酸标记和检测系统。本产品含辣根过氧化物酶（POD）标记抗地高辛抗体的Fab片段。抗地高辛-POD Fab片段用于检测地高辛标记的化合物。
特異性
来自羊的多克隆抗体对于地高辛和异羟洋地黄毒苷有特异性，不会和其他类固醇，比如人雌激素和雄激素反应。
應用
使用抗-地高辛-POD，Fab片段用来在以下实验中检测地高辛-标记化合物:
- 斑点印迹法
- ELISA
- 免疫组织化学
- 原位杂交
- Western印迹法
準備報告
在用地高辛免疫后，山羊IgG通过离心交换色谱进行了纯化，而特异性的IgG通过免疫吸附进行了分离。通过木瓜蛋白酶消化获得的Fab片段经过凝胶过滤，和特异性标记偶联，在缓冲液中稳定。
工作浓度: 偶联剂的工作浓度将取决于应用和底物。以下浓度可作为参考：斑点印迹：150 mU/ml
工作溶液：100mM Tris-HCl,150mM NaCl,pH 7.5.
1% 阻断试剂(w/v), 1到5%热失活胎牛血清(v/v)或羊正常血清，可以用来降低非特异性结合。
工作浓度: 偶联剂的工作浓度将取决于应用和底物。以下浓度可作为参考：斑点印迹：150 mU/ml
- ELISA: 50至150 mU/ml
- 免疫组织化学：250至500 mU/ml
- 原位杂交：1.5至7.5 mU/ml
- Southern blot：150 mU/ml
- Western blot：250至500 mU/ml
工作溶液：100mM Tris-HCl,150mM NaCl,pH 7.5.
1% 阻断试剂(w/v), 1到5%热失活胎牛血清(v/v)或羊正常血清，可以用来降低非特异性结合。
重構
加入1ml双蒸馏水，最终浓度为150 U/ml。
其他說明
仅用于生命科学研究。不可用于诊断。
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訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
does not flash
閃點（°C）
does not flash
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