推荐产品
生物源
sheep
品質等級
共軛
fluorescein conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
lyophilized
包裝
pkg of 200 μg
製造商/商標名
Roche
同型
IgG
儲存溫度
2-8°C
一般說明
抗地高辛-荧光素,Fab片段带有来自多克隆抗地高辛抗体的fab片段,并且与荧光素缀合。
特異性
来自绵羊的多克隆抗体对地高辛配基和地高辛具有特异性,并且与其他类固醇(如人类雌激素和雄激素)没有交叉反应性。
應用
抗地高辛-荧光素,Fab片段可用于检测地高辛标记的化合物:
- 糖结合物研究中地高辛标记的糖
- 荧光 原位 杂交(FISH)
- 免疫组织细胞化学
- 原位 杂交
準備報告
- 工作浓度:结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导:检测糖蛋白中地高辛标记的糖:50至20μg/ml
- 荧光原位 杂交(FISH):1至20μg/ml
- 免疫组织细胞化学:20至50μg/ml
- 原位 杂交:20至50μg/ml
抗DIG抗体具有高度特异性和敏感性。因此,对于FISH,1μg/ml的浓度已足够,并且仅在关键探针的情况下,该浓度可以升高至<10μg/ml(可能产生明显的背景信号)。
工作溶液:溶解
加入1ml双蒸水至终浓度为200μg/ml。
封闭:
PBS,0.5%牛血清白蛋白(w/v),pH7.4。
1%封闭试剂(w/v),1至5%热灭活的胎牛血清(v/v)或绵羊正常血清(可用于减少非特异性结合)。此外,pH可以增加至pH8.5至9.0。
储存条件(工作溶液):每次请制备新鲜溶液!
用地高辛免疫后,通过离子交换层析纯化绵羊IgG,并通过免疫吸附分离特异性IgG。通过木瓜蛋白酶消化获得的Fab片段,可通过凝胶过滤进行纯化,并与特异性标记缀合,并稳定于缓冲液中。
重構
加入1ml双蒸水至终浓度为200μg/ml。
其他說明
仅用于生命科学研究。不可用于诊断。
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訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
does not flash
閃點(°C)
does not flash
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