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H7033

Sigma-Aldrich

PerfectHyb™ Plus杂交缓冲液

for Northern and Southern blotting, solution

别名:

hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

一般說明

Sigma的PerfectHyb Plus是一种即用型杂交缓冲液，适用于Northern和Southern印迹方案。 使用PerfectHyb Plus可将杂交时间缩短至两小时。

應用

可用作Northern印迹、Southern印迹、菌落/噬斑印迹和斑点印迹中所使用的杂交缓冲液。

特點和優勢

  • 快速且易于使用
  • 灵敏度比传统缓冲液高20倍
  • 不需要封闭步骤
  • 与中性和带正电的膜兼容
  • 与化学发光、比色和DIG检测分析兼容
  • 兼容放射性和非放射性探针
  • 与单链或双链DNA、RNA和寡核苷酸探针兼容

其他說明

在溶液中形成的少量沉淀物可以在30-35℃下，通过偶尔轻缓颠倒进行溶解。

法律資訊

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists
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