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Merck
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Key Documents

H7140

Sigma-Aldrich

杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

品質等級

形狀

liquid (2x concentrate)

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

−20°C

一般說明

Sigma的杂交液是一种2倍即用型杂交缓冲液,适用于Northern和Southern印迹方案。

應用

杂交液已用于硝酸纤维素膜的杂交。它也已用于DNA杂交。
适合用作Northern和Southern印迹方案的杂交缓冲液。

成分

Sigma的杂交液含为10倍SSC和2倍Denhardt溶液中的200 μg/mL DNA:
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg聚蔗糖
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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