跳转至内容
Merck

T9285

Sigma-Aldrich

Tris-EDTA 缓冲液

100X concentrate for Northern and Southern blotting

别名:

Tris-EDTA洗涤液, TE 缓冲液 溶液

登录查看公司和协议定价


About This Item

MDL號碼:
分類程式碼代碼:
41105319
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

無菌

0.2 μm filtered

形狀

liquid

pH值

7.8-8.2 (1 × in water)

應用

sample preparation

異物活動

DNase, RNase, none detected

儲存溫度

room temp

SMILES 字串

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI 密鑰

VLEIUWBSEKKKFX-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

一般說明

Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。

應用

TE缓冲液已用于:
  • 分离、重悬或保存DNA和RNA用于PCR或qPCR
  • 凝胶电泳,包括Northern和Southern免疫印迹分析
  • 细菌细胞,用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
  • 免疫组化的抗原修复

成分

TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。

其他說明

For additional information on our range of Biochemicals, please complete this form.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Marika Bogdani et al.
Scientific reports, 7(1), 17231-17231 (2017-12-10)
Cystic fibrosis (CF)-related diabetes (CFRD) is thought to result from beta-cell injury due in part to pancreas exocrine damage and lipofibrosis. CFRD pancreata exhibit reduced islet density and altered cellular composition. To investigate a possible etiology, we tested the hypothesis
Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents.
Espy MJ, et al.
Mayo Clinic Proceedings. Mayo Clinic, 77, 624-628 (2002)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, B-B (1989)
Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
Pak Kin Wong et al.
Analytical chemistry, 76(23), 6908-6914 (2004-12-02)
Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门