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Merck

D2886

Sigma-Aldrich

DNA连接酶 来源于 T4-感染的大肠杆菌

buffered aqueous glycerol solution

别名:

T4 DNA 连接酶, 多核苷酸连接酶, 聚脱氧核糖核苷酸合酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.53

等級

for molecular biology

品質等級

形狀

buffered aqueous glycerol solution

比活性

4,000 U/mL

分子量

68 kDa

UniProt登錄號

儲存溫度

−20°C

基因資訊

bacteriophage T4 ... 30(1258680)

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應用

适用于:
  • 平端和粘性DNA片段的连接
  • 克隆载体和限制性插入片段的连接
  • 双链DNA和RNA或DNA/RNA杂合体中的缺口补平
  • 通过桥连寡核苷酸接头来偶联RNA单链

生化/生理作用

T4 DNA连接酶可在双链体DNA的相邻多核苷酸的末端之间形成能量依赖性的磷酸二酯键。连接反应需要ATP作为辅助因子。平末端片段的连接需要更高的酶浓度并可通过在反应混合物中使用PEG来促进。该酶需要3′羟基和5′磷酸基用于连接。可通过用碱性磷酸酶进行去磷酸化来防止载体DNA的自连。T4连接酶在DNA和RNA缺口的修复中可发挥积极作用。

成分

T4 DNA连接酶以溶于20 mM Tris-HCl(pH 7.5),50 mM KCl,1 mM DTT和50%(v/v)甘油的溶液形式提供。

單位定義

一个Weiss单位的定义是在37℃ 下,在20分钟内催化1 nmol的P32从焦磷酸盐交换至ATP作为Norit可吸收物质所需的酶量。

其他說明

T4 DNA连接酶可通过在65 °C加热10分钟灭活。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析证书(COA)

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P Upcroft et al.
Gene, 51(1), 69-75 (1987-01-01)
We describe a system to generate cDNA or genomic libraries from DNA segments that have blunt termini. Background and rearrangement levels are low, but efficiencies are high and the procedural times very short. T4 ligase in the presence of polyethylene
Tiansheng Wang et al.
Bioorganic & medicinal chemistry letters, 22(11), 3699-3703 (2012-05-09)
A series of 4-amino-pyrido[2,3-d]pyrimidin-5(8H)-ones were designed and synthesized as a novel class of inhibitors of NAD(+)-dependent DNA ligase that possess potency against Gram-positive bacteria.
Tara Sugrue et al.
Stem cells (Dayton, Ohio), 31(1), 137-145 (2012-09-11)
The regeneration of the hematopoietic system following total body irradiation is supported by host-derived mesenchymal stromal cells (MSCs) within the bone marrow. The mechanisms used by MSCs to survive radiation doses that are lethal to the hematopoietic system are poorly
Ayako Nishizawa-Yokoi et al.
The New phytologist, 196(4), 1048-1059 (2012-10-12)
Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the model plant Arabidopsis remains inconclusive. Having established a rapid and highly efficient Agrobacterium-mediated transformation system in rice (Oryza
Athena Kantartzis et al.
Cell reports, 2(2), 216-222 (2012-09-04)
Trinucleotide repeat (TNR) expansions are the underlying cause of more than 40 neurodegenerative and neuromuscular diseases, including myotonic dystrophy and Huntington's disease. Although genetic evidence points to errors in DNA replication and/or repair as the cause of these diseases, clear

实验方案

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

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