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Merck

35845

Sigma-Aldrich

2′,7′-二氯荧光素二乙酸酯

BioReagent, suitable for fluorescence, ≥95% (HPLC)

别名:

DCFHDA, 双氯荧光黄乙酸乙酯

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About This Item

经验公式(希尔记法):
C24H14Cl2O7
CAS号:
分子量:
485.27
Beilstein:
68488
MDL號碼:
分類程式碼代碼:
12352108
PubChem物質ID:
NACRES:
NA.32

產品線

BioReagent

品質等級

化驗

≥95% (HPLC)

mp

232-234 °C (lit.)

溶解度

DMSO: soluble

螢光

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI 密鑰

VQVUBYASAICPFU-UHFFFAOYSA-N

相关类别

一般說明

2′,7′-二氯荧光素二乙酸酯,也称为 DCFHDA 或 Dcfh-DA,是一种敏感的细胞可渗透氧化还原探针。亲脂性非荧光 Dcfh-DA 通过被动扩散和脱乙酰作用很容易穿过细胞膜。脱酰产物是一种对氧化剂敏感的 2′,7′-二氯荧光素(DCHF)。 DCHF 随后被氧化以形成高荧光 DCF,这是被测量的。

應用

2′,7′-二氯荧光素二乙酸酯主要用于检测活性氧(ROS)中间体并量化细胞中的过度 氧化应激 。

Dcfh-DA 用于定量测定,以测量纳米颗粒处理的细胞中的氧化应激。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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