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Merck

MAK143

Sigma-Aldrich

细胞内ROS检测试剂盒(绿色荧光)

sufficient for 200 fluorometric tests (green)

别名:

Fluorometric ROS Detection Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.25

用途

sufficient for 200 fluorometric tests (green)

檢測方法

fluorometric

相關疾病

cancer; neurological disorders

儲存溫度

−20°C

一般說明

活性氧(ROS)的产生是由于有氧呼吸过程中氧的减少和细胞内各种酶系统的作用。在生理水平上,ROS有助于细胞信号传导和宿主防御。超过了生物系统的解毒能力的活性氧产生,导致氧化应激和细胞损伤。细胞的主要损伤是由ROS诱导的大分子的改变,如膜脂、必需蛋白和DNA中多不饱和脂肪酸等。活性氧与阿尔茨海默′s疾病、帕金森病、癌症和衰老等疾病状态有关。

應用

荧光细胞内ROS试剂盒已可用于活性氧(ROS)的生成检测。

適合性

本试剂盒适于通过酶标仪或荧光显微镜检测细胞内的ROS。

原則

荧光细胞内ROS检测试剂盒提供了一种灵敏的、一步荧光检测方法,可在1小时内检测活细胞内的ROS(特别是超氧化物和羟基自由基)。ROS与定位于细胞质的荧光传感器发生反应,产生荧光产物(λex = 490/λem = 520 nm)产物,与ROS含量成正比。该试剂盒可以采用96孔或384孔多孔板形式,并使用荧光显微镜或荧光微孔板读板仪进行读取。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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