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CASP3C

Sigma-Aldrich

Caspase 3 检测试剂盒（比色法）

别名:

Colorimetric Caspase-3 Activity Assay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
 sufficient for 100 standard tests (1 ml)

品質等級

300

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... CASP3(836)

一般說明

Caspase 3 检测试剂盒（比色法）可在细胞溶解产物和半胱天冬酶3纯化制备物样本中快速、有效地检测半胱天冬酶3的活性。

Caspase 3 检测试剂盒（比色法）的检测原理为Caspase 3可水解乙酰基- Asp-Glu-Val-Asp对硝基苯胺（Ac-DEVD-pNA），释放对硝基苯胺（pNA）部分。然后在405 nm处测定对硝基苯胺(εmM=10.5)。再根据405 nm处的吸光度值，就可以计算出底物释放的pDNA浓度。此外，也可以将获得的校准曲线与pNA标准品曲线（试剂盒中包含pNA标准品）进行比较，计算出底物释放的pDNA浓度。

應用

该检测既可采用(a)1 mL的检测体系由分光度计来测定；也可采用(b)100 µL检测体系在96孔板中由ELISA酶标仪来测量。

單位定義

在pH 7.4，25 °C条件下，1单位的Caspase 3每分钟裂解1.0 μmol的Ac-DEVD-pNA。

其他說明

仅限使用17 megohm 水

仅试剂盒组分

产品编号
说明
  • Water (17-18 megaOhm) 125 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • A0835Ac-DEVD-CHO inhibitor .5 mg化学品安全说明书
  • A2559Ac-DEVD-pNA substrate 15 mg化学品安全说明书
  • p-Nitroaniline standard 1 mg

象形圖

Skull and crossbonesHealth hazard
GHS06,GHS08

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點（°F）

415.4 °F - closed cup

閃點（°C）

213 °C - closed cup

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输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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