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Merck

CASP3F

Sigma-Aldrich

胱天蛋白酶3检测试剂盒,荧光法

别名:

Caspase-3 Activity Fluorometric Kit

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

品質等級

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... CASP3(836)

一般說明

胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC),发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线,计算酶解产物AMC浓度(AMC标准品已包含在试剂盒中)。

應用

胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。

單位定義

pH 7.4、25 °C下,每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。

仅试剂盒组分

产品编号
说明

  • Water (17-18 megaOhm) 125 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • A1086Ac-DEVD-AMC substrate 2.5 mg化学品安全说明书

  • A0835Ac-DEVD-CHO inhibitor .5 mg化学品安全说明书

  • A98917-Amino-4-methylcoumarin standard 1 mg化学品安全说明书

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
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Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and
Rebeca Vecino et al.
Scientific reports, 8(1), 1610-1610 (2018-01-27)
Brain preconditioning (PC) refers to a state of transient tolerance against a lethal insult that can be evoked by a prior mild event. It is thought that PC may induce different pathways responsible for neuroprotection, which may involve the attenuation
Ashish Kumar et al.
Scientific reports, 8(1), 2-2 (2018-01-10)
Recently, we have demonstrated that microRNA-31 (miR-31) overexpression is inherent to radiation-induced cell death in the highly radioresistant Sf9 insect cells, and regulates pro-apoptotic Bax translocation to mitochondria. In the present study, we report that at sub-lethal radiation doses for
Joana Silva et al.
BMC complementary and alternative medicine, 18(1), 58-58 (2018-02-16)
Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system. Although the causes of PD pathogenesis remain incomplete, some evidences has suggested that oxidative stress is an important mediator in its pathogenesis. The aim of this study

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