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用途
sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)
品質等級
運輸包裝
dry ice
儲存溫度
−20°C
基因資訊
human ... CASP3(836)
一般說明
胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC),发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线,计算酶解产物AMC浓度(AMC标准品已包含在试剂盒中)。
應用
胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。
單位定義
pH 7.4、25 °C下,每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。
仅试剂盒组分
产品编号
说明
- Water (17-18 megaOhm) 125 mL
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
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