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用途
sufficient for 200 fluorometric tests (Deep red)
應用
cosmetics
food and beverages
檢測方法
fluorometric
相關疾病
neurological disorders; cancer
儲存溫度
−20°C
一般說明
由于在有氧呼吸期间氧气的减少以及细胞内的各种酶系统产生活性氧物质（ROS）。在生理水平，ROS 有助于细胞信号传导和宿主防御。增加的 ROS 产生，高于生物系统的解毒能力，导致氧化应激和细胞损伤。细胞的主要损害是由 ROS 诱导的大分子改变，如膜脂、必需蛋白和 DNA 中的多不饱和脂肪酸。ROS 已经与疾病状态有关，例如阿尔茨海默′病、帕金森′病、癌症和衰老。
應用
- Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.: This article uses fluorometric methods to explore the anti-inflammatory and antioxidative properties of diethyl blechnic, illustrating the role of ROS assays in elucidating the molecular mechanisms underlying the therapeutic effects of natural compounds (Yang S et al., 2019).
適合性
本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。
原則
荧光细胞内 ROS 试剂盒提供灵敏的一步荧光检测法，用于在孵育 1 小时后检测活细胞中的细胞内 ROS（特别是超氧化物和羟基自由基）。ROS 与定位于细胞质的荧光传感器反应，产生与存在的 ROS 量成比例的荧光产物。该测定可以 96 或 384 多孔板形式进行，并且在 λex = 650/λem = 675 nm 处进行检测。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
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