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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
195C, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~167 kDa
物種活性
human
濃度
~1 mg/mL
技術
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... WRN(7486)
一般說明
抗WRN小鼠单克隆抗体(小鼠IgG1亚型)是由小鼠骨髓瘤细胞(p3-NS1/Ag4-1)和用制备自人WRN重组融合蛋白片段进行免疫的BALB/c小鼠脾细胞融合产生的杂交瘤195C生成的。Werner综合征ATP依赖性解旋酶(WRN)位于8号染色体上,为167 kDa。WRN蛋白属于DNA解旋酶的RecQ解旋酶家族。
免疫原
人WRN的重组融合蛋白片段(氨基酸1074-1432)。
應用
WRN抗体还可用于
- 酶联免疫吸附测定(ELISA)
- 免疫印迹
- 免疫沉淀
- 免疫组化
- 蛋白印迹
生化/生理作用
Werner综合征ATP依赖性解旋酶(WRN)蛋白已被证明具有三种已知的催化活性:3′-5′ 解旋酶、核酸外切酶和ATP酶活性。该蛋白的C端区域包含核定位信号(NLS),因此将WRN蛋白靶向细胞核,核仁和核质灶。WRN参与多种DNA代谢过程表明其具有抑癌作用。WRN蛋白的突变会导致Werner综合征(WS)。WRN的表观遗传失活导致WRN-核酸外切酶活性丧失,导致染色体不稳定性增加和对化疗药物的超敏反应。
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Werner complex deficiency in cells disrupts the nuclear pore complex and the distribution of lamin B1
Biochimica et Biophysica Acta - Molecular Cell Research, 1833(12), 3338-3345 (2013)
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(18), 4612-4622 (2016-04-29)
WRN promoter CpG island hypermethylation in colorectal cancer has been reported to increase sensitivity to irinotecan-based therapies. We aimed to characterize methylation of the WRN promoter, determine the effect of WRN promoter hypermethylation upon expression, and validate a previous report
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology, 36(4), 323-325 (2017-08-11)
Werner syndrome is a rare progeroid syndrome caused by the WRN gene mutation. It is characterized by a general appearance of premature aging, diabetes mellitus, and atherosclerosis, and an increased risk of malignancies. We report a patient who presented with
Methods (San Diego, Calif.), 108, 99-110 (2016-05-01)
Functional studies of the roles that DNA helicases play in human cells have benefited immensely from DNA fiber (or single molecule) technologies, which enable us to discern minute differences in behaviors of individual replication forks in genomic DNA in vivo.
Enzymatic and DNA binding properties of purified WRN protein: high affinity binding to single-stranded DNA but not to DNA damage induced by 4NQO
Nucleic Acids Research, 27(17), 3557-3566 (1999)
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