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产品名称
2',7'-二氯荧光素二乙酸酯, ≥97%
品質等級
化驗
≥97%
形狀
powder
儲存溫度
−20°C
SMILES 字串
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI 密鑰
PXEZTIWVRVSYOK-UHFFFAOYSA-N
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一般說明
2′,7′-二氯二氢荧光素二乙酸酯(DCFH-DA)是一种对氧化敏感的荧光染料,用于检测细胞内活性氧(ROS)水平。DCFH-DA是一种细胞渗透性非荧光探针,可被细胞内酯酶水解为DCFH,当被细胞内的ROS氧化时形成荧光DCF。
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
應用
2′,7′-二氯荧光素二乙酸酯适用于:
- 敏感并快速定量活性氧,以响应氧化代谢
- 微孔板法,以检测吞噬细胞中的氧化产物
- 定量多孔髓样分化测定
适用于:敏感并快速定量活性氧,以响应氧化代谢;微孔板法,以检测吞噬细胞中的氧化产物;髓样分化定量多孔测定。
儲存和穩定性
本品应在-20℃下以粉末形式保存。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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