69839-M

T4 DNA Ligase - Novagen

Qualified under the most stringent cloning conditions

• 别名: DNA Ligation
• 分類程式碼代碼: 12352200

属性

• 品質等級: 200
• 製造商／商標名: Novagen^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: dry ice; wet ice
• 儲存溫度: −20°C

说明

一般說明

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application.

警告

Toxicity: Standard Handling

單位定義

4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of ³²P from pyrophosphate into ATP as Norit^®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ Hind III fragments at 16°C in 20 minutes.

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Norit is a registered trademark of Norit N.V.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable