L929 Cell Line from mouse

85011425, mouse adipose tissue, Fibroblast

• 别名: Earles′s Cells, L Cells, NCTC clone 929 Cells
• 分類程式碼代碼: 41106514

属性

• product name: L929 Cell Line from mouse, mouse C3H/An connective tisue, 85011425
• 生物源: mouse adipose tissue
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C3H/An connective tissue

細胞系描述

Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.

應用

Virus studies: PRV, VSV (Indiana), HSV

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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