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product name
MC3T3-E1 Cell Line from mouse, 99072810
生物源
mouse bone
描述
Mouse C57BL/6 calvaria
增長模式
Adherent
染色體組型
Not specified
形態學
Fibroblast-like
產品
Collagen
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse C57BL/6 calvaria
細胞系描述
The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro. Mineral deposits have been identified as hydroxyapatite. Expression of basic fibroblast growth factor (bFGF) mRNA and protein has been shown to be regulated by treatment with TGF β and bFGF. Prostaglandin F2a has been reported to stimulate DNA synthesis and proliferation by up-regulation of insulin-like growth factor I receptors. MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA.
應用
MC3T3-E1 Cell Line has been used to study and propose an actin filament cytoskeleton analysis framework. It has also been used to study the effect of biodegradable magnesium and magnesium alloys on selected properties of these cells stimulated by direct cell/material interaction.
培養基
MEM α + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA 5% CO2 37ºC.
其他說明
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商品
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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