NIH 3T3 Cell Line murine

93061524, mouse embryo, Fibroblast

• 别名: 3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells
• 分類程式碼代碼: 41106514

属性

• product name: NIH 3T3 Cell Line murine, 93061524
• 生物源: mouse embryo
• 描述: Embryonic fibroblast
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse Swiss NIH embryo

細胞系描述

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

應用

DNA transfection studies

NIH 3T3 Cell Line has been used to:

• study calcium-mediated actin reset (CaAR) in response to physiological changes
• study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
• determine the viral titer of the retroviral construct -ST40hTNFα

培養基

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

其他說明

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