293T cell line human

12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth

• 别名: 293T cell line human, 293 T, 293tsA1609neo, 293tsA1609neo Cells, HEK-293-T Cells, HEK-293T Cells, HEK293T Cells, Human Embryonic Kidney 293T Cells
• 分類程式碼代碼: 41106514

属性

• product name: 293T cell line human, 12022001
• 生物源: human kidney (fetal)
• 增長模式: adherent
• 形態學: small slightly rounded cells growing in a dispersed manner
• 產品: SV40 T antigen
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

一般說明

293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line. It expresses the large T antigen of simian virus 40 (SV40).

細胞系描述

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

應用

293T cell line human has been used:

• for lentiviral particle production
• in the expression of eukaryotic proteins
• for small interfering RNA (siRNA) transfection
• for human immunodeficiency virus (HIV) transfection for pseudoviruses production
• as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression

生化／生理作用

Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression. It also possesses stem cell properties. HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.

DNA分析

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培養基

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 10⁴ cells/cm².

其他說明

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