RAW 264.7 Cell Line murine

91062702, Macrophage from blood

• 别名: RAW264 7 Cells, RAW264.7 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: mouse blood
• 增長模式: Semi-adherent
• 染色體組型: Not specified
• 形態學: Macrophage
• 產品: Lysozyme
• 受體: Immunoglobulin, complement
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse monocyte macrophage

細胞系描述

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

應用

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

培養基

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

其他說明

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