J774.2 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011428, mouse blood

• 别名: J774.2 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: mouse blood
• 包裝: tube of 5 μg 85011428-DNA-5UG; pkg of vial of cells 85011428-1VL
• 增長模式: Semi-adherent
• 染色體組型: Not specified
• 產品: Not specified
• 受體: Immunoglobulin, complement
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c monocyte macrophage

細胞系描述

Recloned from J774.1 original ascites and solid tumour. Produces IL-1. Cell line J774.2 was recloned from the original ascites and solid tumor J774.1, and produces interleukin-1 (IL-1).

應用

J774.2 Cell Line from mouse has been used:

• to study the effect of the growth hormone secretagogue peptide A233 on J774.2 cell line
• to study the ability of Mycobacterium tuberculosis clinical isolates to penetrate and grow inside the J774.2 cell line
• to study the effect of ivy leaf dry extract EA 575 on the lipopolysaccharide-induced release of interleukin-6 (IL-6) from J774.2 cell line

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are semi-adherent i.e. some cells grow in suspension , some loosely attach to the surface and others flattened out and attach to the flask. They should be passaged by scraping. Cells should not be allowed to overgrow and become confluent as this can lead to a loss of the flattened adherent cell characteristic.

其他說明

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