3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: 3T3 Swiss Albino, 85022108
• 生物源: mouse embryo
• 增長模式: Adherent
• 染色體組型: 2n = 40; hypertriploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

一般說明

Product has lost contact inhibition

細胞系來源

Mouse Swiss Albino embryo fibroblast

細胞系描述

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

應用

Contact inhibition and virus studies: polyoma and SV40 viruses

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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