B16-F1 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse skin
• 包裝: tube of 5 μg 92101203-DNA-5UG; pkg of vial of cells 92101203-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast-like
• 產品: Melanin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse melanoma, producing melanin

細胞系描述

Mouse melanoma which produces melanin.

應用

B16-F1 cell line from mouse has been used to study the cytotoxic and antioxidant properties of supercritical CO2 extract (SCE) of old man′s beard (Usnea barbata). It has also been used to study the antiproliferative activity of essential oils from Achillea ligustica.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.