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product name
L929(NCTC), 85103115
生物源
mouse adipose tissue
增長模式
Adherent
染色體組型
Not specified
形態學
Fibroblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse C3H/An areolar and adipose tissue
細胞系描述
Derived from normal subcutaneous connective tissue of an adult C3H strain mouse. The cells were treated with 20 methylcholanthrene and produced sarcomas when injected into C3H strain mice.
培養基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split semi-confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells /cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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