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Merck
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Key Documents

Altri documenti

T3934

Sigma-Aldrich

TRI reagent®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

Sinonimo/i:

TRI Reagent® reagente per l’isolamento dell’RNA

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About This Item

Numero MDL:
MFCD00213058
Codice UNSPSC:
12352200
NACRES:
NA.52

impiego

0.75 mL sufficient for 0.25 mL fluid samples

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Descrizione generale

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Applicazioni

TRI Reagent® has been used in RNA extraction.
The reagent has been used for isolating total RNA. The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.
Il Tri reagent consiste in una versione perfezionata del reagente per l'estrazione dell'RNA totale a singolo passaggio sviluppato da Chomczynski. Il metodo di isolamento dell'RNA basato su questo reagente è ampiamente utilizzato e di comprovata affidabilità per le successive applicazioni dell′RNA È ideale per l'isolamento rapido, economico ed efficiente dell'RNA totale o per l'isolamento simultaneo di RNA, DNA e proteine da campioni di origine umana, animale, vegetale, di lievito, batterici e virali.

Caratteristiche e vantaggi

  • Isolamento dell'RNA facilmente scalabile
  • Funziona con campioni di diversa origine: umana, vegetale, di lievito, batterica o virale
  • Ha una resa migliore rispetto ai tradizionali metodi guanidina tiocianato/cloruro di cesio

Note legali

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organi bersaglio

Nervous system,Kidney,Liver,Skin

Rischi supp

EUH032

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

174.2 °F - closed cup

Punto d’infiammabilità (°C)

79 °C - closed cup

Certificati d'analisi (COA)

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Articoli

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.