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P3775

Sigma-Aldrich

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder

Sinonimo/i:

Anti-Protein A

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

fractionated antiserum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Stato

lyophilized powder

Confezionamento

vial of 2 mL lyophilized antiserum

tecniche

indirect ELISA: 1:100,000

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

Immunogeno

Protein A from Staphylococcus aureus

Applicazioni

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.
Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Azioni biochim/fisiol

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Stato fisico

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Ricostituzione

Reconstitute with 2 ml deionized water.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Controllo

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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A fungus-specific outer kinetochore complex, the Dam1 complex, is essential in Saccharomyces cerevisiae, nonessential in fission yeast, and absent from metazoans. The reason for the reductive evolution of the functionality of this complex remains unknown. Both Candida albicans and Schizosaccharomyces
IMMUNOASSAY CONFIGURATIONS
Immunoassay (1996)
Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit
Torres MP, et al.
The Journal of Biological Chemistry, 286(23), 20208-20216 (2011)

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