P2921
Monoclonal Anti-Protein A antibody produced in mouse
clone SPA-27, ascites fluid
Sinonimo/i:
Monoclonal Anti-Protein A
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.46
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
SPA-27, monoclonal
contiene
15 mM sodium azide
tecniche
immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable
Isotipo
IgG1
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
Protein A is a 42 kDa single chain polypeptide isolated from the cell wall of Staphylococcus aureus Cowan I strain.
Specificità
Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.
Immunogeno
protein A from Cowan I strain of Staphylococcus aureus.
Applicazioni
Monoclonal Anti-Protein A antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in western blot and dot blot analyses.
Azioni biochim/fisiol
Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used in these methods either in its native form or conjugated to various markers.
Stato fisico
Monoclonal Anti-Protein A antibody produced in mouse is provided as ascites fluid with 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Dane Vassiliadis et al.
BMC genomics, 20(1), 251-251 (2019-03-30)
Optimal glucose metabolism is central to the growth and development of cells. In microbial eukaryotes, carbon catabolite repression (CCR) mediates the preferential utilization of glucose, primarily by repressing alternate carbon source utilization. In fission yeast, CCR is mediated by transcriptional
F Steindl et al.
Journal of immunological methods, 217(1-2), 143-151 (1998-10-17)
Accuracy of antigen determination in human plasma samples is often adversely affected by immune complex formation between antigens (e.g., HIV-1 p24 protein) and specific antibodies. In this study we describe an optimized method for complete immune complex dissociation (ICD) in
Comparison of protein A affinity sorbents III. Life time study
Hahn R, et al.
Journal of Chromatography A, 1102(1-2), 224-231 (2006)
Matthew B Frankel et al.
Molecular microbiology, 78(1), 238-252 (2010-10-07)
The human pathogen Staphylococcus aureus requires cell wall anchored surface proteins to cause disease. During cell division, surface proteins with YSIRK signal peptides are secreted into the cross-wall, a layer of newly synthesized peptidoglycan between separating daughter cells. The molecular
Rainer Hahn et al.
Journal of chromatography. A, 1102(1-2), 224-231 (2005-12-06)
Protein A affinity chromatography is a popular purification method for immunoglobulins applied at various scales, ranging from micro-tube up to 1000l column format. Three novel high capacity protein A affinity chromatography media have been subjected to a lifetime study using
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