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M4159

Sigma-Aldrich

Blu di metilene

suitable for nucleic acid staining, BioReagent

Sinonimo/i:

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent

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About This Item

Formula empirica (notazione di Hill):
C16H18ClN3S · xH2O
Numero CAS:
122965-43-9
Peso molecolare:
319.85 (anhydrous basis)
Beilstein:
3599847
Numero CE:
200-515-2
Numero MDL:
MFCD00150006
Codice UNSPSC:
12171500
ID PubChem:
57654386
NACRES:
NA.52

Grado

for molecular biology

Nome Commerciale

BioReagent

Forma fisica

powder

tecniche

nucleic acid detection: suitable

Solubilità

water: 1 mg/mL (dark blue)

Compatibilità

suitable for nucleic acid staining

Temperatura di conservazione

room temp

Stringa SMILE

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
WQVSELLRAGBDLX-UHFFFAOYSA-M

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Descrizione generale

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Applicazioni

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Caratteristiche e vantaggi

  • Less hazardous than staining with ethidium bromide
  • Viewable in visible light
  • Fast and easy to use

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H302

Classi di pericolo

Acute Tox. 4 Oral

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Methylene Blue

Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
A C Peacock et al.
Biochemistry, 6(6), 1818-1827 (1967-06-01)
Young-Sharp, D. and Kumar, R.
Technique, 1, 183-183 (1989)
Ariane Felgenträger et al.
BioMed research international, 2013, 482167-482167 (2013-03-20)
Photodynamic inactivation of bacteria (PIB) by efficient singlet oxygen photosensitizers might be a beneficial alternative to antibiotics in the struggle against multiresistant bacteria. Phenothiazinium dyes belong to the most prominent classes of such sensitizers due to their intense absorption in
Angela L Davis et al.
International journal of molecular sciences, 14(2), 4185-4202 (2013-02-23)
Repurposing approved and abandoned non-oncological drugs is an alternative developmental strategy for the identification of anticancer therapeutics that has recently attracted considerable attention. Due to the essential role of the cellular heat shock response in cytoprotection through the maintenance of
Fei Yan et al.
Photochemistry and photobiology, 86(3), 662-666 (2010-02-06)
This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic nanocarriers and nanoconjugates, which may also
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Protocolli

Methylene Blue - Molecular Biology Reagent

Suitable for use in DNA and RNA staining.

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