EMJ human cell line

86021103, IHW Number: 9097

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human blood
• Etnia: North American Caucasian
• Genere: female
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

Consanguineous: No

Homozygous: Yes

Descrizione della linea cellulare

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profilo DNA

A: 0201 0301
Cw: 304
B: 4001
HLA DRA: 102
HLA DRB1: 1302
HLA DRB3: 301
HLA DQAP: 1.4
HLA DQA1: 1021
HLA DQB1: 604
HLA DPA1: 1
HLA DPB1: 0301 0401

Profilo sierologico

HLA A: 2 3
HLA A ief: 2.2 3.2
HLA C: 10
HLA B: 60
HLA B ief: 60
Bw4/bw6: 6
HLA Dw: 19
HLA DR: 13
HLA Dw2: 26
HLA DR52: 52
HLA DQ: 6
HLA DP: 3 4

Terreno di coltura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Mantenimento delle subcolture

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Altre note

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Esclusione di responsabilità

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.