K562 Cell line human

89121407, human blood (chronic myelogenous leukemia), Lymphoblast

• Sinonimo/i: K-562 Cells
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: K562 Cell line human, 89121407, from human blood(chronic myelogenous leukemia)
• Origine biologica: human blood (chronic myelogenous leukemia)
• Modalità di accrescimento: Suspension
• Cariotipo: 2n = 46
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian chronic myelogenous leukaemia

Descrizione della linea cellulare

Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.

Applicazioni

K562 cell line has been used to study the protecting role of cluster of differentiation-39 (CD39) attenuation against DNA damage. It has also been used to study the relevance of a novel sorting approach for separating cells, called “Slice and Dice Sorting”.

Natural killer assay and tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C.

Altre note

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