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Merck
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Key Documents

SLM-240

Sigma-Aldrich

RPMI-1640 complete medium

With ʟ-glutamine and 10% US origin FBS, liquid, sterile-filtered, suitable for cell cutlure

Sinonimo/i:

Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.71

product name

RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS, supplemented with 2mM L-Glutamine and 10% U.S. origin FBS

Livello qualitativo

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Descrizione generale

RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovineserum (FBS). This sterile solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.

Applicazioni

RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)

Qualità

pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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