C9426

3-[(3-colamidopropil)dimetilammonio]-1-propansolfonato

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

• Sinonimo/i: CHAPS
• Formula empirica (notazione di Hill): C₃₂H₅₈N₂O₇S · xH₂O
• Numero CAS: 331717-45-4
• Peso molecolare: 614.88 (anhydrous basis)
• Numero MDL: MFCD00012116
• Codice UNSPSC: 12161902
• ID PubChem: 24892997
• NACRES: NB.22

Proprietà

• Descrizione: zwitterionic
• Livello qualitativo: 200
• Nome Commerciale: BioReagent
• Saggio: ≥98% (HPLC)
• Stato: powder
• PM: 614.88 g/mol (anhydrous basis)
• tecniche: electrophoresis: suitable; protein quantification: suitable
• Impurezze: <0.04% DMF
• Punto di fusione: 157 °C (dec.) (lit.)
• Solubilità: water: 50 mg/mL, clear, colorless
• Compatibilità: suitable for electrophoresis
• Temperatura di conservazione: room temp
• Stringa SMILE: O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1; SJCUTFKCLFLIFE-JWTJKVBLSA-N

Descrizione

Descrizione generale

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Applicazioni

CHAPS has been used:

• For membrane permeabilization of cells
• As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
• As a component in denaturing buffer in enzyme characterization
• As a component of 2D- sample buffer in 2D electrophoresis
• For protein solubilization in isoelectric focusing

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302
• Consigli di prudenza: P264 - P270 - P301 + P312 - P501
• Classi di pericolo: Acute Tox. 4 Oral
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable