A9207
Avidin−Agarose from egg white
aqueous glycerol suspension
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About This Item
Prodotti consigliati
Origine biologica
egg white
Livello qualitativo
Coniugato
agarose conjugate
Forma fisica
aqueous glycerol suspension
Matrice
cross-linked 6% beaded agarose
Attivazione matrice
epichlorohydrin
Gruppi immobilizzati alla matrice
amino
Braccio spaziatore
15 atoms
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
Applicazioni
Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
- in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
- in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
- in avidin bead assay for purification of labelled biotin
- for capture of glycoproteins from murine cornea samples
Azioni biochim/fisiol
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
Definizione di unità
One unit will bind 1.0 μg of d-biotin.
Stato fisico
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Contenuto correlato
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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