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Key Documents

85881

Supelco

Streptavidin, immobilized on Agarose CL-4B

suitable for affinity chromatography

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About This Item

Codice UNSPSC:
12190000
NACRES:
NA.21

Forma fisica

suspension

Livello qualitativo

contiene

0.02% NaN3 as preservative

tecniche

affinity chromatography: suitable

Capacità

≥15 μg/mL, packed gel capacity (biotin)

Temperatura di conservazione

2-8°C

Descrizione generale

streptavidin attached to 4% beaded cross-linked agarose via a C6-spacer (1.2 mg streptavidin/ml packed gel)

Stato fisico

50% suspension in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.15 M sodium chloride

Altre note

Isolation of cell surface glycoprotein by biotinylated concanavalin A and immobilized streptavidin.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves


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Jilian Su et al.
Molecular and cellular probes, 46, 101415-101415 (2019-06-23)
Vascular endothelial growth factor (VEGF) can promote cell division, proliferation and migration. In this study, we aimed to investigate roles of ultrasound-mediated destruction of VEGF-targeted and paclitaxel (PTX)-loaded lipid microbubbles (VTPLLM + US) in human breast cancer cells. The activity of MCF-7 cells
J W Buckie et al.
Analytical biochemistry, 156(2), 463-472 (1986-08-01)
An indirect affinity chromatography procedure utilizing biotinylated lectins and designed for the specific isolation of surface glycoproteins is described. The method is illustrated with intact acute leukemic lymphoblastic cells (ALL cells) with biotin-epsilon-aminocaproyl-concanavalin A (biocap-Con A) and streptavidin-Sepharose 4B. Biocap-Con
Yahui Huang et al.
Clinical & experimental metastasis, 37(4), 477-487 (2020-07-03)
MACC1 (metastasis associated in colon cancer 1) is a key driver that induces metastasis in colon cancer. However, the mechanisms by which MACC1 expression is transcriptionally regulated and the factors enriched at the MACC1 promoter remain largely unknown. The binding
Romina B Cejas et al.
Biological chemistry, 401(9), 1041-1051 (2020-04-17)
Glycosylation is a very frequent post-translational modification in proteins, and the initiation of O-N-acetylgalactosamine (O-GalNAc) glycosylation has been recently described on relevant nuclear proteins. Here we evaluated the nuclear incorporation of a second sugar residue in the biosynthesis pathway of
Yen-Jung Chen et al.
Antioxidants & redox signaling, 31(2), 109-126 (2018-11-13)
Aims: The coordination of neurons to execute brain functions requires plenty of oxygen. Thus, it is not surprising that

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