11426346910
Roche
Anti-Fluorescein-POD, Fab fragments
from sheep
Sinonimo/i:
antibody
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
sheep
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
lyophilized (stabilized)
Confezionamento
pkg of 150 U
Produttore/marchio commerciale
Roche
Temperatura di conservazione
2-8°C
Descrizione generale
Fab fragments from polyclonal anti-fluorescein antibodies, conjugated to horseradish peroxidase. The reagent is an anti-fluorescein antibody, Fab fragments from sheep, conjugated with horse-radishperoxidase (POD). After immunization with fluorescein the sheep IgG (immunoglobulin G) was purified by ion exchange chromatography and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated with POD and stabilized in 60mM Tris-Hepes-buffer, 0.4% bovine immunoglobulin (w/v), 0.2% Germall II (w/v), pH 7.2.
Specificità
The polyclonal antibody reacts with free and bound fluorescein.
Applicazioni
Anti-Fluorescein-POD, Fab fragments are used for detection of fluorescein-labeled compounds in:
- Dot blot
- ELISA (enzyme-linked immunosorbent assay)
- Immunohistocytochemistry
- In situ hybridization
- Southern blot
- Western blot
Nota sulla preparazione
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
- Dot blot: 150 mU/ml
- ELISA: 50 to 150 mU/ml
- Immunohistocytochemistry: 250 to 500 mU/ml
- In situ hybridization: 1.5 to 7.5 U/ml
- Southern blot: 150 mU/ml
- Western blot: 500 to 1000 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Ricostituzione
Add 1 ml double-distilled water to a final concentration of 150 U/ml.
Altre note
For life science research only. Not for use in diagnostic procedures.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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