11426338910
Roche
Anti-Fluorescein-AP, Fab fragments
from sheep
Sinonimo/i:
antibody
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
sheep
Livello qualitativo
Coniugato
alkaline phosphatase conjugate
Forma dell’anticorpo
affinity purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Stato
solution
Confezionamento
pkg of 150 U (200 μl)
Produttore/marchio commerciale
Roche
Isotipo
IgG
Temperatura di conservazione
2-8°C
Descrizione generale
Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)
Specificità
The polyclonal antibody reacts with free and bound fluorescein.
Applicazioni
Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
- Dot blot
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Southern blot
- Western blot
- Electrochemical biosensing technique for the detection of 16S rRNA
Azioni biochim/fisiol
Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).
Caratteristiche e vantaggi
Contents
Solution, stabilized
Solution, stabilized
Nota sulla preparazione
- Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
- ELISA: 150 to 300mU/ml
- Immunohistocytochemistry: 250 to 500mU/ml
- In situ hybridization: 1.5 to 7.5U/ml
- Southern blot: 150mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Altre note
For life science research only. Not for use in diagnostic procedures.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
No data available
Punto d’infiammabilità (°C)
No data available
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