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Merck
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F4648

Sigma-Aldrich

Fast Red TR/Naphthol AS-MX

SIGMAFAST™, fluorogenic, tablet

Sinonimo/i:

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

Numero MDL:
MFCD00132253
Codice UNSPSC:
12352204
NACRES:
NA.83

Nome del prodotto

SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL

Livello qualitativo

200

Stato

tablet

Solubilità

water: 1 tablet/mL, clear to hazy, colorless to faintly yellow

Temperatura di conservazione

−20°C

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Categorie correlate

Applicazioni

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
  • in alkaline phosphatase staining in organ sections
  • for staining chondrogenic spheroids in immunohistochemistry studies
  • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections

Ricostituzione

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.

Note legali

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pittogrammi

CorrosionEnvironment
GHS05,GHS09

Avvertenze

Danger

Indicazioni di pericolo

H314,H411

Classi di pericolo

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLCC3536
SLBT4170
SLBQ5628V
SLBM2372V
SLBH9987V

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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