11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Sinonimo/i:
RNA labeling
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About This Item
Codice UNSPSC:
41105500
Prodotti consigliati
Stato
solution
Livello qualitativo
impiego
sufficient for 20 reactions
Confezionamento
pkg of 40 μL
Produttore/marchio commerciale
Roche
tecniche
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
Temperatura di conservazione
−20°C
Descrizione generale
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Specificità
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Applicazioni
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Qualità
The mix is function tested in combination with the DIG RNA Labeling Kit.
Principio
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Nota sulla preparazione
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
No data available
Punto d’infiammabilità (°C)
No data available
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Articoli
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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