Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

A4541

Sigma-Aldrich

Anti-Biotin−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Anti-Biotin, Anti Biotin Hrp

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Livello qualitativo

Coniugato

peroxidase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

tecniche

direct ELISA: 1:2,000

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Immunogeno

Biotin-KLH conjugate

Applicazioni

Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.
Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Nota sulla preparazione

Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Pittogrammi

Exclamation markEnvironment

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Katarzyna Kwiatkowska et al.
Journal of cell science, 116(Pt 3), 537-550 (2003-01-01)
Activation of Fcgamma receptor II (FcgammaRII) induces rearrangement of the actin-based cytoskeleton that serves as a driving force for FcgammaRII-mediated phagocytosis and FcgammaRII capping. To get insight into the signaling events that lead to the actin reorganization we investigated the
Samuel H Henager et al.
Nature methods, 13(11), 925-927 (2016-11-01)
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-terminal cysteine (N-Cys)-containing peptides, but the requirement of a Cys residue at the ligation junction can limit the utility of
Ee Lin Wong et al.
Nature communications, 10(1), 66-66 (2019-01-10)
Protein-templated fragment ligations have been established as a powerful method for the assembly and detection of optimized protein ligands. Initially developed for reversible ligations, the method has been expanded to irreversible reactions enabling the formation of super-additive fragment combinations. Here
Damien Destouches et al.
The Journal of biological chemistry, 287(52), 43685-43693 (2012-10-31)
Blockage of the metastasis process remains a significant clinical challenge, requiring innovative therapeutic approaches. For this purpose, molecules that inhibit matrix metalloproteinases activity or induce the expression of their natural inhibitor, the tissue inhibitor of metalloproteinases (TIMPs), are potentially interesting.
Nataliya Gorinski et al.
The Journal of biological chemistry, 295(18), 5970-5983 (2020-03-19)
Barttin is the accessory subunit of the human ClC-K chloride channels, which are expressed in both the kidney and inner ear. Barttin promotes trafficking of the complex it forms with ClC-K to the plasma membrane and is involved in activating

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.