L040050

Cap A

configured for (ÄKTA^® and OligoPilot^®)

• Codice UNSPSC: 12352200
• NACRES: NA.21

Proprietà

• Tipo: for DNA synthesis
• Livello qualitativo: 200
• Nome Commerciale: Proligo Reagents
• Forma fisica: liquid
• Condizioni di stoccaggio: dry at room temperature
• Impurezze: ≤100 ppm water content (Karl Fischer)
• Colore: clear
• Compatibilità: conforms to structure (GC, Formulation); configured for (ÄKTA^® and OligoPilot^®)
• Temperatura di conservazione: room temp

Descrizione

Applicazioni

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Nota sulla preparazione

Acetonitrile / N-Methylimidazole 8/2 (v/v)

Altre note

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

Note legali

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS06,GHS08,GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H225,H302 + H332,H311,H314,H361d
• Consigli di prudenza: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 35.6 °F
• Punto d’infiammabilità (°C): 2 °C