CS0170
Thioredoxin Reductase Assay Kit
1 kit sufficient for 100 assays (1 mL)
Synonym(e):
Thioredoxin Reductase Activity Kit
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)
About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Qualitätsniveau
Verwendung
kit sufficient for 100 assays (1 mL)
Versandbedingung
dry ice
Lagertemp.
−20°C
Angaben zum Gen
human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)
Verwandte Kategorien
Allgemeine Beschreibung
The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
Anwendung
Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).
Leistungsmerkmale und Vorteile
- An easy and simple method
- The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
- The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
- The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Assay Buffer 5x for Thioredoxin Reductase 30 mL
- Thioredoxin Reductase 10 μg
- Thioredoxin Reductase Inhibitor Solution .05 mL
- 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
- NADPH 25 mg
- Dimethyl Sulfoxide (DMSO) 7.5 mL
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Repr. 2
Lagerklassenschlüssel
10 - Combustible liquids
Flammpunkt (°F)
188.6 °F
Flammpunkt (°C)
87 °C
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